• Fika Dewi Pratiwi Bangka Belitung University
  • Eka Sari




Depuration, Blood clams, Total of Bacteria


Blood clams cultured in Sukal Village West Bangka Regency sell in the market around Bangka Belitung Island Province. These blood clams often sell without sanitary and hygiene control treatment during cultivation or post-harvest. For that reason, the clams have the potential for pathogenic bacterial contamination that can harm the consumer's health. The study aimed to analyze the initial content of total bacteria (total plate count) in blood clam sample collected from Sukal Village and analyze the content of total bacteria (TPC) after depuration treatment for 24 hours and 48 hours. The depuration experiment followed the recirculating system for 24 hours and 48 hours. The UV light used to irradiate the natural saline water and chitosan from shrimp shell used as a filter and antibacterial agent. The temperature and salinity were constant at 29 o C and 30 o/oo. Total plate count (TPC) method used in this research according to SNI 01-2332.3-2006, while ISO 4833-1: 2013; PerKa BPOM No. 16 used as the quality standard of total bacteria content in blood clam. The result found that the blood clams collected from the first station contained total bacteria exceeded the established quality standards. Independent T-test showed that there was no significant difference between the reduction in the TPC content of blood clam samples with a depuration time of 24 hours and a time of 48 hours.


Author Biography

Fika Dewi Pratiwi, Bangka Belitung University

Manajemen Sumberdaya Perairan



Afiati, N, “The Ecology of Two Species of Blood Clams Anadara granosa (L.) And Anadara Antiqua (L.) In Central Java, Indonesia,†Disertasi. University of Wales. Bangor. United Kingdom. 1994.



semakin-diminati. 8 Agustus 2019

Dinas Kelautan dan Perikanan, “Rencana Strategis Dinas Kelautan dan Perikanan 2017-2022,†Provinsi Kepulauan Bangka Belitung. 2017

Lee, R. A, Lovatelli dan L. Ababouch, “Bivalve Depuration: Fundamental and Practical Aspects,†Food and Agriculture Organization of The United Nations. ISBN 978-92-5-106006-3. Rome.pp.161. 2008.

Barile N.B, M Scopa, E Nerone, G Mascilongo, S Recchi, S Cappabianca dan L Antonetti, “ Study of the Efficacy of a Closed Cycle Depuration System on Bivalve Molluscs,†Vetenaria Italiana. Vol.45, No.4, hal.555-566. 2009.

Badan Standarisasi Nasional, “ Cara Uji Mikrobiologi-Bagian 3:Penentuan Angka Lempeng Total (ALT) pada produk perikanan,†SNI 01-2332.3-2006. Badan Standarisasi Nasional. Jakarta. 2006.

Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia, “PerKa BPOM No.16 tahun 2016. Kriteria Mikrobiologi,†2016.

Ekawati E.R dan S N H Yusmiati, “Detection of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa),†International Symposium on Food and Agro-biodiversity (ISFA) 2017. IOP Conf. Series: Earth and Environmental Science 102. 012086. 2018.

1. Corrales, L.A.V, C.M Vindas, O.P Prieto dan G.Z Calero, “Preliminary Assessment of Small Scale Bacterial Depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica. Rev. Mar. Cost. ISSN 1659-455X, Vol.5, hal.107-117. 2013.

Terence O, B. Mark, S. Mary, M. Daniel, O. Michael dan R. Cillian, “ Depuration centre management. Course notes,†ARCHIMER. Ifremer's institutional repository. 1995.

McLeod C, D.Polo, J.C Le Saux dan F.S. Le Guyader, “Final Report: Evaluating the effectiveness of depuration in removing norovirus from oysters,†Seafood Safety Assessment Ltd. and the French Research Institute for Exploitation of the Sea. 2017

Sorio, J dan J. Peralta, “Evaluation of a Small Scale UV-treated Recirculating Depuration System for Oysters (Crassostrea iredalei),†International Conference on Fisheries and Aquatic Sciences. 2018

Ahmed S, M.Ahmad dan S. Ikram, “Chitosan: A Natural Antimicrobial Agent- A Review,†Journal of Applicable Chemistry, Vol.3, No.2, hal. 493-503. 2014

Otwell W.S, G.E Rodrick dan R.E Martin, “Molluscan Shellfish Depuration,†CRC Press. Boca Raton. 398 p. 1991.

Lee R, Lovatelli, A. dan Ababouch, L, “Depuración de bivalvos: aspectos fundamentales y prácticos,†FAO Documento Técnico de Pesca No. 511. Rome, Italy: FAO. 2010.

Cusson, M, R Tremblay, G. Daigle, dan M Roussy, “Modeling the depuration potential of blue mussels (Mytilus spp.) in response to thermal shock,†Aquaculture, Vol.250, hal. 183-193, 2005

Love, D. C, G. L Lovelace, dan M. D. Sobsey, “Removal of Escherichia coli, Enterococcus fecalis, coliphage MS2, poliovirus, and hepatitis A virus from oysters (Crassostrea virginica) and hard shell clams (Mercinaria mercinaria) by depuration,†Int. J. Food Microbiol., Vol. 143, hal. 211-217, 2010.

1. Oliveira, J., Cunha, A., Castilho, F., Romalde, J. L. dan Pereira, M. J, “ Microbial contamination and purification of bivalve shellfish: Crucial aspects in monitoring and future perspectives-A mini review,†Food Control, Vol. 22, No.6, hal. 805-816. 2011