UJI DAYA HAMBAT EKSTRAK Chaetoceros calcitrans TERHADAP BAKTERI Aeromonas salmonicida

Authors

  • Mr. Maftuch Brawijaya University
  • Febby Hadi Setyawan Program Studi Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya Malang
  • Heny Suprastyani

DOI:

https://doi.org/10.21776/ub.jfmr.2018.002.01.6

Keywords:

Aeromonas salmonicida, Chaetoceros calcitrans, Uji daya hambat

Abstract

The impact of use antibiotic can cause problems on aquaculture such as bacterial resistance and residues for consumer. Therefore, one of alternatives that can be used to inhibit or kill bacteria, one of which is the use of plankton Chaetoceros calcitrans. This research aims to know the influence of the granting C. calcitrans extract that can inhibit or kill bacteria and know the best dose for inhibit A. salmonicida bacteria. The experiment method  used randomize completely design consist of four treatment and two control. A treatment (0.3 ppm), B treatment (15.3 ppm), C treatment (30.3 ppm),  D treatment (45.3 ppm), positive control (add chlorampenicol 5ppm) and negative control (without extract). The result of research showed the highest average of clear zone diameter in D treatment 5.37 mm and the lowest average of clear zone diameter in A treatment 2.89 mm, with linear equation y = 3.2067 + 0.0509x and coefficients R2 = 0.9262. The relationship between C. calcitrans extract in inhibitng gwoth of A. salmonicida bacteria showed that the average diameter of the clear zone has increase in line with the addition of extracts. Form the research showed that the extract of C. calcitrans was bacteriostatic (inhibits the growth of bacteria) and best obtained at the treatment dosage of D with a dose of 45.3 ppm and included into the category of middle.

References

Agustin, D. W. 2005. Perbedaan khasiat antibakteri bahan irigasi antara hidrogen peroksida 3% dan infusum daun Sirih 20% terhadap bakteri mix. Maj. Ked. Gigi. (Dent. J.). 38(1): 45–47

Ashari, C., R. A. Tumbol dan M. E. F. Kolopita. 2014. Diagnosa penyakit bakterial pada Ikan Nila (Oreocrhomis niloticus) yang di budidaya pada jaring tancap di Danau Tondano. Budidaya Perairan. 2(3): 24-30.

Darsana, I. G. O., I. N. K. Besung dan H. Mahatmi. 2012. Potensi daun Binahong (Anredera cordifolia (tenore) Setenis) dalam menghambat pertumbuhan bakteri e.coli secara in vitro. Indonesia Medicus Veterinus. 1(3): 337-351.

Fadhilla, R., E. A. P. Iskandra dan H. D. Kusumaningrum. 2012. Aktivitas antibakteri ekstrak tumbuhan Rumput Lumut Hati (Marchantia paleacea) terhadap bakteri patogen dan perusak pangan. J. Teknologi dan Industri Pangan. 23(2): 126-131.

Muqsith, A. 2013. Aktivitas antibakteri ekstrak fenol Gracillaria verrucosa terhadap bakteri Aeromonas salmonicida secara in vitro. Samkia. 3(1): 69-75.

Pan, X., F. Chen, T. Wu, H. Tang and Z. Zhao. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Jurnal Food Control. 1(20): 598-602.

Pontoh, O. 2012. Analisa usaha budidaya ikan dalam jaring apung di Desa Tandengan Kecamatan Eris Kabupaten Minahasa, Sulawesi Utara. Pacific Journal. 2(7): 1424-1428.

Putri, R. W., W, Tjajaningsih dan D, Handijatno. 2008. Daya antibakteri pigmen pyocyanin dari isolate Pseudomonas aeruginosa terhadap Aeromonas hydrophila secara in vitro. Jurnal berkala ilmiah perikanan. 3(1): 65-73.

Rinawati, N. D. 2011. Daya antibakteri Tumbuhan Majapahit (Crescentia cujete L.) terhadap bakteri Vibrio alginolyticus. Fak. Matematika dan Ilmu Pengetahuan Alam. ITS Surabaya. 1-13.

Seraspe, E. B., B. F. Ticar, M. J. Formacion, I. G. Pahila, M. R. de la Pena and E. C. Amar. 2012. Antibacterial properties of the microalgae Chaetoceros calcitrans. Asian Fisheries Science. 25: 343-356

Seraspe, E. B., S. Gabotero, M. R. De la Peña, I. G. Pahila and E.C. Amar. 2014. Evaluation of dietary freeze-dried Chaetoceros calcitrans supplementation to control Vibrio harveyi infection on Penaeus monodon juvenile. Aquaculture 432. 212–216.

Setyaningsih, I ., L. Hardjito, D. R. Monintja, M. F. A. Sondita, M. Bintang, N. Lailati dan L. Panggabean. 2017. Ekstraksi senyawa antibakteri dari diatom Chaetoceros calcitrans dengan berbagai metode. Jurnal Biologi Indonesia. 5(1): 23-33.

Shannon, E . and N. Abu-Ghannam. 2016. Antibacterial derivatives of marine algae: an overview of pharmacological mechanisms and applications. Marine drugs. 14(81): 1-23.

Sumardjo, D. 2008. Pengantar Kimia : Buku Panduan Kuliah Mahasiswa Kedokteran dan Program Strata I Fakultas Bioeksakta. Jakarta. EGC. 650hlm

Sumino, A. Supriyadi dan Wardiyanto. 2013. Efektivitas ekstrak daun ketapang (Terminalia cattapa L.) untuk pengobatan infeksi Aeromonas salmonicida pada ikan patin (Pangasioniodon hypophthalmus). Jurnal Sain Veteriner. 3(1): 79-88.

Wiyanto,D,B. 2010. Uji aktivitas antibakteri ekstrak Rumput Laut Kappaphycus alvarezii dan Eucheuma denticullatum terhadap Bakteri Aeromonas hydrophila dan Vibrio harveyii. Jurnal Kelautan. 3(1): 1-17.

Downloads

Published

2018-04-10

Issue

Section

Articles

Most read articles by the same author(s)