Antibakteri Ekstrak Etanol Serbuk Kering Sargassum cristaefolium Terhadap Bakteri Escherischia coli dan Salmonella thyposa
Keywords:Phaeophyta dried powder, antibacterial, Escherischia coli, Salmonella thyposa
Sargassum cristaefolium dried powder can be used as food and fed additives as well as Â a beverage powder, contained some minerals and terpenoid active ingredients. This study aimed to determine the anti-bacterial activity of Sargassum cristaefolium dried powder on the inhibition of Escherischia coli and Salmonella thyposa bacteria in vitro. The research showed that the dried powder of Sargassum cristaefolium had better inhibition on Salmonella thyposa (2.3 mm) compared to Escherischia coli (1.2 mm) although it was classified as weak bactericidal. Sargassum cristaefolium dried powder showed positive testing for alkaloids, flavonoids, terpenoids and tannins and does not contain saponins, had low toxicity (LC50 322 ppm). The LCMS test showed a peak at 2.16 retention time with a molecular weight of 339.39 m/z. The structure of the Sargassum cristaefolium antibacterial active ingredient should be clarified using C-NMR and H-NMR.
Zubia, M., Payri, C. and Deslandes, E. â€œAlginate, mannitol, phenolic compounds and biological activities of two range-extending brown algae, Sargassum mangarevense and Turbinaria ornata (Phaeophyta: Fucales), from Tahiti (French Polynesia)â€. J. Appl.Phycol vol 20, no 6, hal.1033-1043 .Journal of Applied Phycology, 20(6), pp.1033-1043. 2008
Salosso, Y. and Jasmanindar, Y. â€œDiversity of brown macroalgae in Kupang Bay waters and alginate content potential and its phytochemistryâ€. Aquacult Aquarium Conserv Legislation vol 11 no 3 hal 598-605. 2018
Sugiono, S., Masruri, M., Estiasih, T. and Widjarnako, S.B. â€œStructural and Rheological Characteristics of Alginate from Sargassum cristaefolium Extracted by Twin Screw Extruderâ€. J.Aquat Food Prod Technol Journal of Aquatic Food Product Technology, pp.1-16. October 2, 2019
Sudaryono, A., Haditomo, A.H. and Isnansetyo, A.. â€œEvaluation of dietary supplementation of aqueous extract of brown algae Sargassum cristaefolium on growth performance and feed utilization of juvenile white shrimp Litopenaeus vannameiâ€. AACL Bioflux, vol 8 no 2 2015
Isnansetyo, A., Fikriyah, A. and Kasanah, N. â€œNon-specific immune potentiating activity of fucoidan from a tropical brown algae (Phaeophyceae), Sargassum cristaefolium in tilapia (Oreochromis niloticus)â€ Aquac Int vol 24 no 2 hal 465-477. .2016
Wu, G.J., Shiu, S.M., Hsieh, M.C. and Tsai, G.J.â€Anti-inflammatory activity of a sulfated polysaccharide from the brown alga Sargassum cristaefolium.â€ Food Hydrocolloloids vol 53 hal 16-23. 2016
Wang, C.Y., Wu, T.C., Hsieh, S.L., Tsai, Y.H., Yeh, C.W. and Huang, C.Y. â€œAntioxidant activity and growth inhibition of human colon cancer cells by crude and purified fucoidan preparations extracted from Sargassum cristaefoliumâ€. J Food Drug Anal.vol 23 no 4 hal 766-777 .2015
Borines, M.G., De Leon, R.L. and McHenry, M.P. â€œBioethanol production from farming non-food macroalgae in Pacific island nations: Chemical constituents, bioethanol yields, and prospective species in the Philippines.â€ Renewable & Suatainable Energy Rev.vol 15 no 9 hal 4432-4435. 2011
Balanquit, B.J.R. and Fuentes, R.G. â€œPreliminary Phycochemical Screening and Antioxidant Activity of Some Brown Algae Sargassum Species From Lawaan, Eastern Samarâ€.J. Nat Stud vol 14 no 1 hal 12-21. 2015
Lailiyah, A., Adi, T.K. and Yusnawan, E. â€œKapasitas Antioksidan dan Kandungan Total Senyawa Fenolik Ekstrak Kasar Alga Coklat Sargassum cristaefolium dari Pantai Sumenep Maduraâ€. Alchemy hal 18-30. 2014
Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis) that it is not under consideration for publication elsewhere; that if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.