In Vitro Anti-Vibrio Activity of Miana Leaves (Coleus scutellarioides (L) Benth)
DOI:
https://doi.org/10.21776/ub.jfmr.2024.008.01.7Keywords:
Antibacterial Activity Test, Chloroform Fraction, Inhibition Zone, VibrioAbstract
The aim of this research is to determine the bioactive compounds of miana leaves of Coleus scutellarioides (L) Benth against Vibrio spp.. This research was carried out from April to August 2018 at the Fish Pests and Diseases Laboratory, Department of Fisheries, Gadjah Mada University. Miana leaf samples were dried at room temperature, macerated with ethanol solvent and tested for antibacterial activity. Separation of bioactive compounds was carried out using liquid partition and silica gel using the column chromatography method. MIC (minimum inhibitory concentration) is applied to evaluate antibacterial activity. The results showed that the highest antibacterial activity was found in the chloroform fraction with inhibitory zone diameters for V. parahaemolitycus, V. alginolitycus, and V. harveyii of 25 mm, 26 mm, and 20 mm at a dose of 20 µg/disk. These findings indicate that the antibacterial activity of miana leaf crude extract is comparable to oxytetracycline with inhibition zones of 25 mm, 31 mm and 20 mm respectively against similar bacterial strains at 20 µg/disk. Therefore, miana leaves are a prospective natural source of anti-vibrio compounds.
References
FAO. 2014 The state of world fisheries and aquaculture: opportunities and challenges. FAO Fisheries and Aquaculture Departement. Food and Agriculture Organization of the United Nations. Rome
Austin, B., and D.A. Austin. 2007. Bacterial fish pathogens: disease of farmed and wild fish, 4 th Ed. Springer Praxis Publishing, Chichester, United Kingdom, 552 pp
Feliatra, Zainurinand Dessy Yoswaty, 2014. Phatogenity of Vibrio sp on Tiger Prawn (Penaeus monodon). 1 (2). 23-36 [in Indonesian]
Wei, Q., 2002. Social and economic impacts of aquatic animal health problems in aquaculture in China. In: Arthur JR, Philips MJ, Subasinghe RP, Reantaso MB, and MacRae IH. (Eds). Primary Aquatic Animal Health Care in Rural, Small-Scale, Aquaculture Development. FAO Fish Technical Paper No. 406. FAO. 55-61pp
Dhayanithi, N.B., Ajith Kumar., T.T., Arockiaraj, J., Balasundaram, C., Harikrishnan, R., 2015. Dietary supplementation of Avicennia marina extract on immune protection and disease resistance in Amphiprion against Vibrio alginolyticus. Fish & Shelfish Immunology. 45, 52-58
Rosyidah, Cicik., 2008 Tamarine Tamarindus Indica L as biocoagulant on water quality from physical chemical, and bacteriology. Thesis, School of Postgraduate, Department of Biology, Faculty of Science and Technology. Malang: Universitas Islam Negeri Malang. [in Bahasa Indonesia]
Ridwan Y., Darusman K.L., Satrija F., Andaryani E., 2006 the chemical compound of painted nettle (Coleus blumei Benth) leave extract and its anthelmintic activity against chicken tapeworm. Jurnal III Pertanian Indonesia. 11 (2) : 1-6 [in Bahasa Indonesia]
Ridwan Y., 2010 Anticestoda activity on miana leaves Coleus blumei Benth on Hymenolepis microstoma taken from mice. Dissertation. School of Postgraduate, Bogor Agricultural University. Bogor. [in Bahasa Indonesia]
Palette T., 2017. Effects of Miana Leaves (Coleus Scutellarioides (L) Benth) Extract on Mycobacterium Tuberculosis in inducted Mus musculus using Mycobacterium under intraperitonium condition. Dissertation. School of Postgraduate of Medical Science. Universitas Hasanuddin. Makassar.
Saptiani G., Prayitno S.B., and Anggoro S., 2013 Antibacterial extract derived from Acanthus Ilicifolius on vibrio Harveyi In Vitro [Potensi Antibakteri Ekstrak Daun Jeruju (Acanthus ilicifolius) Terhadap Vibrio harveyi Secara In Vitro]. Jurnal Kedokteran Hewan. 7,(1):17-21 [in Bahasa Indonesia]
Purnama R., Melki EP W.A., and Rozirwan, 2010 Potensial of Halimedha renchii Seaweed Extract as Antibacterial Vibrio sp. [Potensi Ekstrak Rumput Laut Halimedha rencii sebagai Antibakteri Vibrio sp.]. Maspari Jurnal 02 (2011) 82-88.
Hidhayati N., 2017 Extraction, Fractions, and Characterization of Antibacterial Compound derived from Mangrove Leaves Ceriops Decandra (Griff.) Ding Hou. Thesis. School of Postgraduate in Universitas Gadjah Mada. Yogyakarta. 65p [in Bahasa Indonesia]
Azis, 2019 Antibacterial Analysis of Dragon Scales Leaves under invitro condition (Drymoglossum Pilosellaoides) on Vibrio harveyi and Vibrio parahaemolyticus. Journal of Aquaculture and Fish Health. 8 (2). 86-91 [in Indonesian]
Hamdillah A., Isnansetyo A., Istiqomah I., Puspita I.D., Handayani D.P., dan Kaneko T., 2019 Antibacterial Activity of Coastal Plants and Marine Sponges from Kei Island Indonesia against Bacterial Fish Pathogens. Pharmacognosy Journal, 11 (4), 812-817
Harbone J B. 1987. Phytochemical Methods, A Guide to Modern Ways of Analyzing Plants. Translation. K. Padmawinata and Soediro. Publisher: ITB. Bandung.
Tiwari P., Kumar B., Kaur M. Kaur G., Kaur H., 2011. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, Vol. 1, Issue 1; 98-106.
Hamdillah A., Isnansetyo A., Istiqomah I., Puspita I.D., Handayani D.P., Kaneko T., 2019. Antibacterial Activity of Coastal Plants and Marine Sponges from Kei Island Indonesia against Bacterial Fish Pathogens. Pharmacogn Journal; 11(4):812-817.
Isnansetyo A., Kamei Y., 2009 Anti-methicillin-resistant Staphylococcus aureus (MRSA) activity of MC21-B,an antibacterial compound produced by the marine bacterium Pseudoalteromonas phenolica O-BC30T. International Journal of Antimicrobial Agents. 34 (2) : 131-135
Wall P.E., 2005 Thin-layer chromatography a modern practical approach. The Royal Society of Chemistry. 176p
Rosamah E., 2019. Thin layer chromatography, a simple method for the chemical analysis of woody plants. Mulawarman University Press. 69p.
Cahyono B., Suzery M., 2020. Natural material separation methods (Theoretical and experimental aspects). Kompas Ilmu, 117p.
Horikawa M., T. Noro, Y. Kamei, 1999. In vitro anti-methicillin resistant Staphylococcus aureus activity found in extract of marina algae indigenous to the coastline of Japan. J. Antibiotics. 52(2):186-9
Miller R.A., Walker R.D., Baya A, Clemens K., Coles M., 2003. Antimicrobial Susceptibility Testing of Aquatic Bacteria: Quality control Disk Diffusion Ranges for Escherichia coli ATCC 25922 and Aeromonas Salmonicida Subsp Salmonicida ATCC 33658 at 22 and 28 0C. J. Clin. Microbiol. 41:4318-4323
Isnansetyo A., Kamei Y., 2005. Direct antagonistic Method for screening anti-methicillin resistant Staphylococcus aureus (MRSA) substance, producing marine bacteria. Biota. 10 (3): 141-145
Achmad, S.A., 1986 Organic Chemical from Nature 1 [Kimia Organik dari Alam 1]. Penerbit Karunika Jakarta, Universitas Terbuka. 148p [in Indonesian]
Meiriana Y., 2006 Effects of Red Fruit (Pandanus conoideus Lam) on Proliferation of Human Lymphocyte Cell under invitro condition, Thesis, School of Postgraduate of Institut Pertanian Bogor. [in Indonesian]
Wiedosari E. 2007 Role of Natural Immunomodulator (Aloe Vera) in Cellular and Humoral Immune Responses. Indonesian Bulletin of Animal and Veterinary Sciences. 17 (4): 165-171 ;in Bahasa Indonesia]
Wahyuniarti I., Soesatyo M.H.N.E., Ghufron M., Yustina, Sumiwi A.A., Wiryawa S., 2009 Red fruit oil increases the lymphocyte proliferation activity of mice after infection Listeria Monocytogeneses [Minyak Buah Merah Meningkatkan Aktivitas Proliferasi Limfosit Limpa Mencit Setelah Infeksi Listeria Monocytogenesis]. Jurnal veteriner, 10 (3) : 143-149 [in Bahasa Indonesia]
Goodman, L. S. and Gilman, A. 1960. The Pharmacological Basis of Therapeutics. New York: MacMillan Company. 1831p.
Choi, J.M., Lee, E.O., Lee, H,Y., 2007 Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytother Res. 21, 954-959
Panda, S., Jafri, M., Kar, A., and Meheta, B.K., 2009 Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Fitoterapia, 80(2):123-680
Gabay, O., Sanchez, C., Salvat, C., 2010 Stigmasterol: a phytosterol with potential anti osteoarthritic properties. Am J Clin Nutr, 18, 106-116.
Azis, 2019. In Vitro Analysis of Dragon Scale Leaves (Drymoglossum pilosellaoides) Antibacterial Activity Against Vibrio harveyi and Vibrio parahaemolyticus Bacteria. Journal of Aquaculture and Fish Health Vol. 8(2);86-91.
Hidayati S., Lumbessy S.Y., dan Azhar F., 2021. Antibacterial Activity of Kitolod Leaves Ethanol Extract (Isotoma longiflora) Against Bacteria Vibrio sp. Reason Vibriosis In Vaname Shrimp (Litopenaeus vannamei). Bioscientist : biological scientific journal. Vol. 9(1);86-95.
Purnama R., Melki, Putri W.A.E., Rozirwan, 2011. Potential of Halimeda renchii and Euchema cottonii Seaweed Extracts As an Antibacterial Vibrio sp. Maspari Journal, 02;82-88.
Lisdawati V., Mutiatikum D., Alegantina S., Astuti Y.N., 2008 Characterization of miana leaves (Plectranthus Scutellarioides (L.) Bth.), and piper betle L.) Using physical and chemical obtained from Locally Ingredients for Anti-malaria North Sulawesi. (4) 18, 213-225
Promosiana, A., N. Indartiyah, and M. P Tahir. 2007 Mapping on Bioregional Biopharmacy plant [Peta Potensi. Bioregional Tanaman Biofarmaka]. Direktorat Budidaya Tanaman Sayuran dan Biofarmaka, Dirjen Hortikultura, Deptan RI. Jakarta 73-91pp [in Bahasa Indonesia]
Salituro, G.M. and Dufresne, C., 1998 Isolation by Low Pressure Column Chromatography, In R. J. P. Cannell, ed. Methods in Biotechnology Vol 4, Natural Products Isolation, Totowa, New Jersey: Humana Press Inc.
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