IDENTIFIKASI BAKTERI PADA LARVA IKAN TUNA SIRIP KUNING (Thunnus albacares)
DOI:
https://doi.org/10.21776/ub.jfmr.2020.004.01.1Keywords:
Thunus albacares, larva, bakteriAbstract
Perbenihan tuna sirip kuning (Thunus albacares) telah dilakukan sejak tahun 2015 di Balai Besar Riset Budidaya Laut dan Penyuluhan Perikanan, Gondol, Bali. Akan tetapi, dalam perbenihan tuna sirip kuning masih dihadapkan adanya tingkat kematian yang tinggi pada tahap larva. Penelitian ini bertujuan untuk mengidentifikasi bakteri pada perbenihan larva tuna sirip kuning. Bakteri diisolasi dari larva umur 5, 10, 15, 20 hari setelah menetas pada media marine agar. Koloni bakteri yang tumbuh direisolasi berdasarkan morfologinya. Setiap isolat diidentifikasi secara molekuler berdasarkan urutan nukleotida dalam gen ribosom 16S menggunakan pasangan primer 27F dan 1492R. Kesamaan masing-masing urutan nukleotida dianalisis dengan BLAST. Hasil penelitian menunjukkan bahwa ditemukan 9 isolat bakteri dari larva ikan tuna sirip kuning yang tumbuh dengan morfologi berbeda. Hasil Analisa BLAST, bakteri tersebut memiliki kemiripan dengan Bacillus cereus, B. methylotrophicus, B. amyloliquefaciens, Pseudomonas plecoglossicida, P. putida dan Vibrio sp. strain voc. Hasil penelitian ini diharapkan dapat menjadi informasi awal tentang bakteri yang ada pada larva ikan tuna sirip kuning.References
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