EFEK PROBIOTIK Bacillus megaterium PTB 1.4 DAN Pediococcus pentosaceus E2211 TERHADAP REPONS IMUN DAN KELANGSUNGAN HIDUP IKAN LELE (Clarias sp.) SELAMA UJI TANTANG Aeromonas hydrophila

Muhammad Subhan Hamka, Anja Meryandini, Widanarni Widanarni, Ardana Kurniaji
  JFMR, pp. 567-577  

Abstract


Penyakit bakterial pada kegiatan budidaya ikan sudah menjadi masalah yang sering dihadapi pembudidaya. Salah satu jenis bakteri yang menyebabkan penyakit pada ikan lele (Clarias sp.) adalah bakteri Aeromonas hydrophila. Tujuan penelitian ini untuk mengevaluasi efek pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap respons imun dan kelangsungan hidup ikan lele selama uji tantang dengan bakteri A. hyrophila. Probiotik yang digunakan adalah kombinasi 0,5% B. megaterium PTB 1.4 dan 0,5%  P. pentosaceus E2211. Pemberian probiotik dilakukan pada hari ke-0 hingga hari ke-30 melalui pakan. Uji tantang dilakukan pada hari ke-30 hingga hari ke-40. Hasil penelitian menunjukkan bahwa pemberian probiotik dapat berpengaruh terhadap respons imun dan kelangsungan hidup ikan lele dalam menekan infeksi A. hydrophila. Total eritrosit, hemoglobin, hematokrit, total leukosit, aktivitas fagositik dan aktivitas lisozim signifikan (P<0,05) teramati meningkat pada hari ke-35 dan menurun pada hari ke-40. Kelangsungan hidup ikan lele berhasil dipertahankan pada 87,62±4,83%. Total bakteri A. hydrophila meningkat pada hari ke-35 dan menurun pada hari ke-40. Pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada dosis 0,5% dapat menekan pertumbuhan bakteri A. hydrophila dan meningkatkan status kesehatan ikan lele.

Keywords


Aeromonas hydrophila, kelangsungan hidup, probiotik, respons imun

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References


Adeyemo, B. T., & Solomon, S. G. (2014). Haematological reference ranges of cultured Clarias gariepinus in the Lower Benue River Basin , Nigeria. Comparative Clinical Pathology, February 2018. https://doi.org/10.1007/s00580-012-1624-1

Ahmed, S. M., & Shoreit, A. A. M. (2001). Bacterial haemorrhagic Septicemia in Oreochromis niloticus at aswan fish hatcheries (pp. 190–206).

Alamanda, I. E., Handajani, N. S., & Budiharjo, A. (2007). Penggunaan metode hematologi dan pengamatan endoparasit darah untuk penetapan kesehatan ikan lele dumbo (Clarias gariepinus) di kolam budidaya Desa Mangkubumen Boyolali. Biodiversitas, 8, 34–38.

Alipin, K., & Sari, T. A. (2020). Indikator kesehatan ikan kerapu cantik (Epinephelus sp.) yang terdapat pada budidaya keramba Pantai Timur Pangandaran. Metamorfosa Journal of Biological Sciences, 7(September), 285–292. https://doi.org/10.24843/metamorfosa.2020.v07.i02.p18

Anderson, D. P., & Siwicki, A. K. (1993). Basic hematology and serology for fish health programs (pp. 185–202). Fish Health Section, Asian Fisheries Society, Manila, Phillipines.

Arief, M., Fitriani, N., & Subekti, S. (2014). Pengaruh pemberian probiotik berbeda pada pakan komersial terhadap pertumbuhan dan efisiensi pakan ikan lele sangkuriang (Clarias sp.). Jurnal Ilmiah Perikanan Dan Kelautan, 6(1), 5.

Astria, Q., Nuryati, S., Nirmala, K., & Alimuddin, A. (2017). Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus. Jurnal Akuakultur Indonesia, 16(2), 154. https://doi.org/10.19027/jai.16.2.154-163

Austin, B., & Austin, D. A. (2012). Bacterial Fish Pathogens. Springer International Publishing Switzerland, 49(0), 6221.

Blaxhall, P. C., & Daisley, K. W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5(6), 771–781. https://doi.org/10.1111/j.1095-8649.1973.tb04510.x

Cruz, P. M., Ibáñez, A. L., Hermosillo, O. A. M., & Saad, H. C. R. (2012). Use of Probiotics in Aquaculture. ISRN Microbiology, 2012, 1–13. https://doi.org/10.5402/2012/916845

Fowoyo, P. T., & Achimugu, F. (2019). Virulence of Aeromonas hydrophila isolated from fresh water catfish. Journal of Biosciences and Medicines, 7, 1–12. https://doi.org/10.4236/jbm.2019.71001

Hamka, M. S., Meryandini, A., & Widanarni. (2020). Growth performance and immune response of catfish Clarias sp . given probiotics Bacillus megaterium PTB 1 . 4 and Pediococcus pentosaceus E2211 Kinerja pertumbuhan dan respons imun ikan lele Clarias sp . yang diberi probiotik Bacillus megaterium PTB 1 . 4. Jurnal Akuakultur Indonesia, 19(1), 50–60. https://doi.org/10.19027/jai.19.1.50-60

Hamtini, Widanarni, & Meryandiri, A. (2015). Isolasi dan seleksi Bacillus sp. dan ikan lele (Clarias sp.) serta potensinya sebagai probiotik. Jurnal Biologi Indonesia, 11(1), 11–19.

Hanif, A., Bakopoulos, V., & Dimitriadis, G. J. (2004). Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae. Fish and Shellfish Immunology, 17(5), 411–435. https://doi.org/10.1016/j.fsi.2004.04.013

Hardi, E. H. (2018). Bakteri patogen pada ikan air tawar Aeromonas hydrophila dan Pseudomonas fluorescens. Mulawarman University Press.

Hardi, E. H., Harris, E., Lusiastuti, A. M., Perairan, L. M., Mulawarman, U., & Timur, K. (2011). Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe β -hemolitik dan Non-hemolitik pada Ikan Nila. Jurnal Veteriner, 12(2), 152–164.

Hardi, E. H., Pebrianto, C. A., Hidayanti, T., & Handayani, R. T. (2014). Infeksi Aeromonas hydrophila melalui jalur yang berbeda pada ikan nila (Oreochromis niloticus ) di loa kulu kutai kartanegara kalimantan timur. Jurnal Kedokteran Hewan, 8(2).

Harley, J. P., & Prescott, L. M. (2002). Laboratory Exercises in Microbiology. 5th Edition. The McGraw-Hill Companies.

Indriyanti, A., Julyantoro, P. G. S., & Wijayanti, N. P. P. (2021). Protection of Thiophenone in Catfish (Clarias sp.) Larvae When Challenged with Aeromonas hydrophila. Advances in Tropical Biodiversity and Environmental Sciences, 5(1), 23. https://doi.org/10.24843/atbes.2021.v05.i01.p04

Janda, J. M., Abbott, S. L., Janda, J. M., & Abbott, S. L. (2010). The genus Aeromonas: taxonomy, pathogenicity , and infection. Clinical Microbiology Reviews, 23(1). https://doi.org/10.1128/CMR.00039-09

Kartikaningsih, H., Yahya, Rohman, F., & Jaziri, A. (2020). Characteristics of Aeromonas hydrophila- infected Catfish ( Clarias sp .). IOP Conf. Series: Earth and Environmental Science, 493. https://doi.org/10.1088/1755-1315/493/1/012036

Kurniaji, A., Nuryati, S., Murtini, S., & Alimuddin. (2018). Maternal immunity response and larval growth of anti cyhv-3 dna vaccinated common carp (Cyprinus carpio) at different pre-spawning time. Pak. J. Biotechnol., 15(3), 689–698.

Lusiastuti, A. M., Sumiati, T., & Hadie, W. (2013). Probiotik Bacillus firmus untuk pengendalian penyakit Aeromonas hydrophila pada budidaya ikan lele dumbo, Clarias gariepinus. Jurnal Riset Akuakultur, 8, 253–264.

Mansyur, A., & Tangko, M. (2008). Probiotik: pemanfaatannya untuk pakan ikan berkualitas rendah. Media Akuakultur, 3(2), 145–149.

Mesalhy, S. (2013). A Review of Fish Diseases in the Egyptian Aquaculture. Cgiar, July, 1–41. www.livestockfish.cgiar.org

Mulia, D. S. (2007). Keefektivan vaksin Aeromonas hydrophila untuk mengendalikan penyakit MAS (motile aeromonas septicemia) pada gurami (Osphronemus gouramy Lac.). Jurnal Pembangunan Pedesaaan, 7(1), 43–52.

Munaeni, W., Yuhana, M., & Widanarni, W. (2014). Effect of Micro-encapsulated Synbiotic at Different Frequencies for Luminous Vibriosis Control inWhite Shrimp (Litopenaeus vannamei). Microbiology Indonesia, 8(2), 73–80. https://doi.org/10.5454/mi.8.2.5

Nayak, S. K. (2010). Probiotics and immunity: A fish perspective. Fish and Shellfish Immunology, 29(1), 2–14. https://doi.org/10.1016/j.fsi.2010.02.017

Öztürk, R. Ç., & Altinok, I. (2014). Bacterial and viral fish diseases in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 14(1), 275–297. https://doi.org/10.4194/1303-2712-v14_1_30

Pȩkala-Safińska, A. (2018). Contemporary threats of bacterial infections in freshwater fish. Journal of Veterinary Research (Poland), 62(3), 261–267. https://doi.org/10.2478/jvetres-2018-0037

Prayogi, Y. T., Kusdarwati, R., & Kismiyati. (2016). Isolasi, identifikasi dan presentasi ikan lele dumbo (Clarias gariepinus) yang terinfeksi bakteri Aeromonas hydrophila yang dipelihara di keramba jaring apung di Bozem Moro Krembangan, Surabaya. Journal of Aquaculture and Fish Health, 5(2).

Purwanti, S., Suminto, & Sudaryono, A. (2014). The description of blood profile catfish Clarias gariepinus that is fed with a combination of artificial feed and earthworm Lumbricus rubellus. Journal of Aquaculture Management and Technology, 3, 53–60.

Rahmaningsih, S., Zenuddin, M., & Sudianto, A. (2018). Gambaran hematokrit darah ikan lele sangkuriang (Clarias gariepinus) yang diberi pakan serbuk daun majapahit (Cresentia cujete L) dan diinfeksi dengan bakteri Aeromonas hydrophila. Jurnal Kelautan Dan Perikanan Terapan, 1(2), 63–67.

Rosyidah, E., Meryandini, A., & Sunarti, T. C. (2014). The Use of Lactic Acid Bacteria and Cellulolytic Bacteria to Improve the Chemical Properties of Corn Flour. MAKARA of Science Series, 17(3), 75–80. https://doi.org/10.7454/mss.v17i3.2944

Sahoo, P., Paul, A., Sahoo, M., Pattanayak, S., P, R. K., & Bk, D. (2018). Incidences of Infectious Diseases in Freshwater Aquaculture Farms of Eastern India : A Passive Surveillance based Study from 2014-2018. 1–5. https://doi.org/10.35248/2155-9546.20.10.579

Secombes, C. J. (1990). Isolation of salmoid macrophages and analysis of their killing activity (pp. 137–155). Di dalam: Stolen JS, Fletcher TC, Anderson DF, Roberson BS, von Mulsvinkel WB, editor. Tehniques in fish immunology Vol 1. SOS Publications, Fair Haven.

Sukenda, Jamal, L., Wahjuningrum, D., & Hasan, A. (2008). Penggunaan kitosan untuk pencegahan infeksi Aeromonas hydrophila pada ikan lele dumbo Clarias sp. Jurnal Akuakultur Indonesia, 7(2), 159–169.

Tarigan, N., Meiyasa, F., Efruan, G. K., Sitaniapessy, D. A., & Pati, D. U. (2019). Aplikasi probiotik untuk pertumbuhan ikan lele (Clarias batrachus) di kelurahan malumbi, sumba timur. Jurnal Mitra, 3(1), 50–57.

Tizard, I. (2004). An Introduction Veterinary Immunology. Edisi ke-7. Elsevier Health Sciences.

Triyaningsih, Sarjito, & Prayitno, S. B. (2014). Patogenitas Aeromonas hydrophila yang diisolasi dari ikan lele dumbo (Clarias gariepinus) yang berasal dari Boyolali. Journal of Aquaculture Management and Technology, 3, 11–17.

Turnip, E. R., Widanarni, W., & Meryandini, A. (2018). Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp. Jurnal Akuakultur Indonesia, 17(1), 68. https://doi.org/10.19027/jai.17.1.68-80

Uribe, C., Folch, H., Enriquez, R., & Moran, G. (2011). Innate and adaptive immunity in teleost fish : a review. Veterinarni Medicina, 56(January), 486–503. https://doi.org/10.17221/3294-VETMED

Wedemeyer, G. A., & Yasutake, W. T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health (p. 18 p.). U.S. Fish and Wildlife Service.

Zhang, D., Xu, D., & Shoemaker, C. (2016). Experimental induction of motile Aeromonas septicemia in channel catfish ( Ictalurus punctatus ) by waterborne challenge with virulent Aeromonas hydrophila. Aquaculture Reports, 3, 18–23. https://doi.org/10.1016/j.aqrep.2015.11.003

Zubaidah, A., Samsundari, S., & Hidayaturrahmi. (2019). Effectiveness of strychnine bush extract (Strychnos ligustrina Blume) on the prevalence and survival rate of Dumbo catfish (Clarias gariepinus) infected by Aeromonas hydrophila. Indonesian Journal of Tropical Aquatic, 2(1), 1–8. https://doi.org/10.22219/ijota.


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